CAJ | 학술논문

目的：观察肺泡灌洗治疗支气管感染的临床疗效,并探讨IL-17、IL-8、IL-4等炎症因子在支气管哮喘发病中的作用。方法随机选择该院2013年1月—2014年1月收治的30例支气管哮喘急性发作期患者纳入观察组,另选30例性别,年龄,病程资料相同的患者,进行常规治疗纳入对照组,观察组患者采用纤维支气管镜下肺泡灌洗术治疗,观察治疗后的PaO2、SaO2和PaCO2改善情况及临床疗效；同时采集所有研究对象的支气管肺泡灌洗液(BALF),采用多抗体夹心酶联免疫吸附方法检测2组BALF中IL-17、IL-8、IL-4的含量。结果经支气管肺泡灌洗治疗后,观察组总有效率为96.6%,PaO2和SaO2均较治疗前升高,差异有统计学意义(P<0.05),PaCO2较治疗前降低,差异有统计学意义(P<0.05)；观察组BALF上清液中IL-17、IL-8的水平较正常对照组升高,差异有统计学意义(P<0.05)。结论支气管肺泡灌洗可显著改善支气管哮喘患者气道功能,临床疗效显著。 IL-17、IL-8可能介导了支气管哮喘患者急性发作期的发作。
목적：관찰폐포관세치료지기관감염적림상료효,병탐토IL-17、IL-8、IL-4등염증인자재지기관효천발병중적작용。방법수궤선택해원2013년1월—2014년1월수치적30례지기관효천급성발작기환자납입관찰조,령선30례성별,년령,병정자료상동적환자,진행상규치료납입대조조,관찰조환자채용섬유지기관경하폐포관세술치료,관찰치료후적PaO2、SaO2화PaCO2개선정황급림상료효；동시채집소유연구대상적지기관폐포관세액(BALF),채용다항체협심매련면역흡부방법검측2조BALF중IL-17、IL-8、IL-4적함량。결과경지기관폐포관세치료후,관찰조총유효솔위96.6%,PaO2화SaO2균교치료전승고,차이유통계학의의(P<0.05),PaCO2교치료전강저,차이유통계학의의(P<0.05)；관찰조BALF상청액중IL-17、IL-8적수평교정상대조조승고,차이유통계학의의(P<0.05)。결론지기관폐포관세가현저개선지기관효천환자기도공능,림상료효현저。 IL-17、IL-8가능개도료지기관효천환자급성발작기적발작。
Objective To observe the clinical efficacy of bronchoalveolar lavage in the treatment of bronchial asthma and investi-gate the role of inflammatory cytokines, such as IL-17, IL-8, IL-4 in the pathogenesis of bronchial asthma. Methods 30 patients with acute exacerbation of bronchial asthma admitted in our hospital from January 2013 to January 2014 were enrolled into obser-vation group, and other 30 patients with the same gender, age, and data of course of disease were enrolled into the control group. Patients in the observation group were treated by bronchoalveolar lavage with fiberbronchoscope, and the control group was treated by the conventional treatment. The improvement of PaO2, SaO2 and PaCO2 and clinical efficacy were observed after treatment. Lev-els of IL-17, IL-8, IL-4 in bronchoalveolar lavage fluid (BALF) of all subjects were collected and detected with multi- antibody sandwich ELISA. Results After the bronchoalveolar lavage treatment, in the observation group, the total effective rate was 96.6%, PaO2 and SaO2 increased significantly(P<0.05) and PaCO2 decreased significantly compared with pre-treatment(P<0.05); the levels of IL-17 and IL-8 in BALF supernatant in the observation group were significantly higher than those in the control group(P<0.05). Conclusion Bronchoalveolar lavage can significantly improve the airway function in patients with bronchial asthma with significant clinical effect. IL-17, IL-8 may mediate the acute exacerbation of bronchial asthma attack.