CAJ | 학술논문

利用一例肺鳞癌手术标本通过原代培养建立了肺癌细胞系命名为HB-99。该细胞系呈单层贴壁生长，从相差显微镜和电镜分析具有细胞的多形性，细胞倍增时间为24小时，克隆形成率40%，染色体改变复杂，众数63-65。将细胞移植到裸鼠体内而生长的肿块具有与原始病人手术标本相似的组织形态。免疫组织化学分析，近100%的细胞表达角蛋白17（CK17），10%的细胞表达波形蛋白（vimentin）。根据该细胞系的生物学特征提示HB-99是一新建立的肺鳞癌细胞系。
이용일례폐린암수술표본통과원대배양건립료폐암세포계명명위HB-99。해세포계정단층첩벽생장，종상차현미경화전경분석구유세포적다형성，세포배증시간위24소시，극륭형성솔40%，염색체개변복잡，음수63-65。장세포이식도라서체내이생장적종괴구유여원시병인수술표본상사적조직형태。면역조직화학분석，근100%적세포표체각단백17（CK17），10%적세포표체파형단백（vimentin）。근거해세포계적생물학특정제시HB-99시일신건립적폐린암세포계。
We have established a human lung squamous carcinoma cell line ,designed HB-99, by culturing primary tumor sample. The cells of HB-99 derived from resected specimen of a male patient with lung squamous cancer. They grew in monolayers and showed cellular morphology by phase contrast and electronic microscopy. The HB-99 cells had a doubling time of 24 hours and a cloning efficiency of 40%. Chromosomal analysis showed complicated rearrangements with a modal number of 63～65. When hetero-transplanted to nude mice, HB-99 grew to form tumor with the same morphology as the original one from the patient. The results of immunohistochemistry suggested that CK17 expressed in almost all cells while only 5%～10% cells had Vimentin. HB-99 is really a newly established cell line of lung squamous carcinoma.