CAJ | 학술논문

采用丝网印刷技术制作的一次性薄层碳电极具有良好的分析性能。经化学修饰二茂铁后，以丝素为固定化载体固定含有多酚氧化酶的蘑菇萃取蛋白，所得酶电极对其底物邻苯二酚和多巴胺有良好响应，线性范围为2.0×10-8～2.0 × 10-2mol/L，在30s内即可达到95％稳态电流。该电极的RSD为2.7％，且可保持10天以上的工作寿命。该酶电极在低温湿润环境中可保存较长时间，因此有较好的商品化前景。
채용사망인쇄기술제작적일차성박층탄전겁구유량호적분석성능。경화학수식이무철후，이사소위고정화재체고정함유다분양화매적마고췌취단백，소득매전겁대기저물린분이분화다파알유량호향응，선성범위위2.0×10-8～2.0 × 10-2mol/L，재30s내즉가체도95％은태전류。해전겁적RSD위2.7％，차가보지10천이상적공작수명。해매전겁재저온습윤배경중가보존교장시간，인차유교호적상품화전경。
This paper reports the technique to develop a disposable screen-printing base carbon electrode for the utilization in the study of enzyme electrode. The silk fibroin was applied to immobilize the mushroom extracted protein, which contained abundant polyphenol oxidase, on the surface of base electrode when it was chemically moldified by ferrocene. The voltammetric current of this enzyme electrode responded to the concentration of substrate such as catechol or dopamine. The linear range is from 2.0× 10-8 mol/L to 2.0× 10-2 mol/L, it reaches to a 95%steady value of current within less than 30 seconds, and the RSD equals to 2.7%. The service life of this enzyme electrode is at least 10 days and it will be preserved for a longer period at a humid and cool condition. All of these performances of the enzyme electrode make clear that its commercial development would be very possible.