CAJ | 학술논문

目的：将传统中医辨证方法与现代系统生物学理论相结合，研究2型糖尿病中医辨证分型及虚实证与基于超高效液相色谱法（UPLC）-四极杆飞行时间（QTOF）-质谱（MS）/MS的血清代谢组学的关系。方法选择2013年6月至2014年4月住院并确诊为2型糖尿病患者90例（辨证分为阴虚热盛、气阴两虚、血淤气滞3种证型）。应用UPLC-QTOF-MS/MS方法测定健康者及不同中医证型2型糖尿病患者血清内源性代谢物，并用偏最小二乘-判别式分析（PLS-DA）代谢谱。结果 PLS-DA分析结果表明，该法不仅可有效区分疾病组和健康对照组，也可明显区分患者中阴虚热盛、气阴两虚、血淤气滞3种证型。结论基于UPLC-QTOF-MS/MS和模式识别的代谢组学技术在揭示传统中医理论本质及对2型糖尿病的临床研究方面具有广泛的应用前景。
목적：장전통중의변증방법여현대계통생물학이론상결합，연구2형당뇨병중의변증분형급허실증여기우초고효액상색보법（UPLC）-사겁간비행시간（QTOF）-질보（MS）/MS적혈청대사조학적관계。방법선택2013년6월지2014년4월주원병학진위2형당뇨병환자90례（변증분위음허열성、기음량허、혈어기체3충증형）。응용UPLC-QTOF-MS/MS방법측정건강자급불동중의증형2형당뇨병환자혈청내원성대사물，병용편최소이승-판별식분석（PLS-DA）대사보。결과 PLS-DA분석결과표명，해법불부가유효구분질병조화건강대조조，야가명현구분환자중음허열성、기음량허、혈어기체3충증형。결론기우UPLC-QTOF-MS/MS화모식식별적대사조학기술재게시전통중의이론본질급대2형당뇨병적림상연구방면구유엄범적응용전경。
Objective To study the relationship between the traditional Chinese medicine syndrome differentiation and excess or deficiency syndrome in type 2 diabetes mellitus (T2DM) with the serum metabolomics based on UPLC-QTOF-MS/MS by combining the traditional Chinese medicine differentiation method with the modern systematic biological theory. Methods 90 inpatients diagnosed as T2DM in our hospital from June 2013 to April 2014 were selected and divided into 3 kinds of syndrome type including the type of Yin deficiency and intense heat ,type of deficiency of both Qi and Yin and type of Qi stagnancy and blood stasis. The endogenous metabolites in the healthy individuals and different Chinese medicine syndrome types of T2DM were detected by the UPLC-QTOF-MS/MS method. Then the metabolic profiling was analyzed by using the partial least squares dis-criminant(PLS-DA) method. Results The PLS-DA analysis results showed that this method not only effectively distinguished the disease group from the healthy control group,but also differentiated the three syndrome types of Yin deficiency and intense heat, deficiency of both Qi and Yin,and Qi stagnancy and blood stasis in T2DM patients. Conclusion The metabolomic technique based on UPLC-QTOF-MS/MS and pattern recognition has the extensive application prospects in revealing the essence of tradi-tional Chinese medicine and clinical research of T2DM.