中外医疗
中外醫療
중외의료
China Foreign Medical Treatment
2015年
24期
176-177
,共2页
肌酸激酶同工酶%免疫抑制法%胶体金法
肌痠激酶同工酶%免疫抑製法%膠體金法
기산격매동공매%면역억제법%효체금법
CK-MB%Immune suppression method%Colloidal gold method
目的：对肌酸激酶同工酶（CK-MB）的两种测定方法进行比较，分别为免疫抑制法和胶体金法。方法标本来自于2014年12月-2015年2月期间在该院住院的患者，主要针对儿科住院患者送检的标本,年龄段在6个月~3岁，随机选取152例。在全自动生化分析仪上分别进行测定肌酸激酶（CK）和肌酸激酶同工酶(CK-MB)活性，并从中选取二者不成比例的标本进一步用胶体金方法测定CK-MB质量。结果测定标本共152人份，CK在正常范围内，CK-MB升高，高于正常值（51±20）U/L（免疫抑制法结果），用胶体金法结果为（2.367±1.038）ng/mL；CK高于正常值时，CK-MB免疫抑制法结果为（73±30）U/L，胶体金法结果为（9.152±1.123）ng/mL。结论CK-MB的活性测定一般均为免疫抑制法，但由于方法学的局限性，可引起测定结果假性升高，出现异常升高时，及时和临床取得联系，可结合患者的病情，进一步用胶体金法测定CK-MB的质量，为患者提供更准确的检测结果。
目的：對肌痠激酶同工酶（CK-MB）的兩種測定方法進行比較，分彆為免疫抑製法和膠體金法。方法標本來自于2014年12月-2015年2月期間在該院住院的患者，主要針對兒科住院患者送檢的標本,年齡段在6箇月~3歲，隨機選取152例。在全自動生化分析儀上分彆進行測定肌痠激酶（CK）和肌痠激酶同工酶(CK-MB)活性，併從中選取二者不成比例的標本進一步用膠體金方法測定CK-MB質量。結果測定標本共152人份，CK在正常範圍內，CK-MB升高，高于正常值（51±20）U/L（免疫抑製法結果），用膠體金法結果為（2.367±1.038）ng/mL；CK高于正常值時，CK-MB免疫抑製法結果為（73±30）U/L，膠體金法結果為（9.152±1.123）ng/mL。結論CK-MB的活性測定一般均為免疫抑製法，但由于方法學的跼限性，可引起測定結果假性升高，齣現異常升高時，及時和臨床取得聯繫，可結閤患者的病情，進一步用膠體金法測定CK-MB的質量，為患者提供更準確的檢測結果。
목적：대기산격매동공매（CK-MB）적량충측정방법진행비교，분별위면역억제법화효체금법。방법표본래자우2014년12월-2015년2월기간재해원주원적환자，주요침대인과주원환자송검적표본,년령단재6개월~3세，수궤선취152례。재전자동생화분석의상분별진행측정기산격매（CK）화기산격매동공매(CK-MB)활성，병종중선취이자불성비례적표본진일보용효체금방법측정CK-MB질량。결과측정표본공152인빈，CK재정상범위내，CK-MB승고，고우정상치（51±20）U/L（면역억제법결과），용효체금법결과위（2.367±1.038）ng/mL；CK고우정상치시，CK-MB면역억제법결과위（73±30）U/L，효체금법결과위（9.152±1.123）ng/mL。결론CK-MB적활성측정일반균위면역억제법，단유우방법학적국한성，가인기측정결과가성승고，출현이상승고시，급시화림상취득련계，가결합환자적병정，진일보용효체금법측정CK-MB적질량，위환자제공경준학적검측결과。
Objective To compare the two measure methods of Creatine kinase isoenzyme (CK-MB), and they were immunosup-pression method and colloidal gold method respectively. Methods Specimens were from randomly selected 152 patients hospital-ized in our hospital from December 2014 to February 2015,mainly for pediatric inpatients aged 6 months to 3 years. Using auto biochemical analyzer to measure the activity of creatine kinase (CK) and creatine kinase isoenzyme (CK-MB), and selected those disproportionate specimens for further measure of CK-MB using colloidal gold method. Results Measured 152 specimens in total, and CK was within the normal range, but CK-MB was higher than normal (51±20) U/L(using immune suppression method), while using colloidal gold method the result was (2.367±1.038) ng/mL;When CK was higher than normal, the immune suppression mea-sure results of CK-MB was (73±30) U/L, and the result of colloidal gold method was (9.152±1.123) ng/mL. Conclusion The mea-surement of CK-MB activity generally are immune suppression method, but due to the limitation of the methodology, it can cause pseudo rise. When encounter abnormal rise, make contact with clinical doctors, taking the patient's state into account, one could use colloidal gold method for further measure of the quality of CK-MB, in order to provide more accurate measure results for pa-tients.
