遗传
遺傳
유전
Hereditas
2015年
9期
885-898
,共14页
高通量测序%转座子%偏好性%多态性%水平转移
高通量測序%轉座子%偏好性%多態性%水平轉移
고통량측서%전좌자%편호성%다태성%수평전이
high throughput sequencing%transposable element%bias%polymorphism%horizontal transfer
高通量测序技术极大地提高了测序效率,大幅度降低了测序成本,同时该技术具有特异性好、灵敏度高、精确性高等优势,目前已被广泛应用于遗传变异、转录组学和表观组学等研究。近年来,高通量测序技术也逐渐应用于转座子的研究,并取得了丰硕的成果。本文主要综述了高通量测序技术在转座子研究中的应用,包括转座子含量估算、靶点偏好性及分布、多态性及群体频率、稀有转座子的鉴定、转座子的水平转移以及转座子标签技术中的应用等,并简要介绍了目前研究中采用的主要测序策略和算法,及其存在的利弊和相应的解决方案。最后对高通量测序技术,尤其是第三代测序技术的发展趋势和它们在转座子未来的研究中的应用进行了展望,以期为相关的科研人员提供一个全面的了解和参考。
高通量測序技術極大地提高瞭測序效率,大幅度降低瞭測序成本,同時該技術具有特異性好、靈敏度高、精確性高等優勢,目前已被廣汎應用于遺傳變異、轉錄組學和錶觀組學等研究。近年來,高通量測序技術也逐漸應用于轉座子的研究,併取得瞭豐碩的成果。本文主要綜述瞭高通量測序技術在轉座子研究中的應用,包括轉座子含量估算、靶點偏好性及分佈、多態性及群體頻率、稀有轉座子的鑒定、轉座子的水平轉移以及轉座子標籤技術中的應用等,併簡要介紹瞭目前研究中採用的主要測序策略和算法,及其存在的利弊和相應的解決方案。最後對高通量測序技術,尤其是第三代測序技術的髮展趨勢和它們在轉座子未來的研究中的應用進行瞭展望,以期為相關的科研人員提供一箇全麵的瞭解和參攷。
고통량측서기술겁대지제고료측서효솔,대폭도강저료측서성본,동시해기술구유특이성호、령민도고、정학성고등우세,목전이피엄범응용우유전변이、전록조학화표관조학등연구。근년래,고통량측서기술야축점응용우전좌자적연구,병취득료봉석적성과。본문주요종술료고통량측서기술재전좌자연구중적응용,포괄전좌자함량고산、파점편호성급분포、다태성급군체빈솔、희유전좌자적감정、전좌자적수평전이이급전좌자표첨기술중적응용등,병간요개소료목전연구중채용적주요측서책략화산법,급기존재적리폐화상응적해결방안。최후대고통량측서기술,우기시제삼대측서기술적발전추세화타문재전좌자미래적연구중적응용진행료전망,이기위상관적과연인원제공일개전면적료해화삼고。
High throughput sequencing technology has dramatically improved the efficiency of DNA sequencing, and decreased the costs to a great extent. Meanwhile, this technology usually has advantages of better specificity, higher sensitivity and accuracy. Therefore, it has been applied to the research on genetic variations, transcriptomics and epigenomics. Recently, this technology has been widely employed in the studies of transposable elements and has achieved fruitful results. In this review, we summarize the application of high throughput sequencing technology in the fields of transposable elements, including the estimation of transposon content, preference of target sites and dis-tribution, insertion polymorphism and population frequency, identification of rare copies, transposon horizontal transfers as well as transposon tagging. We also briefly introduce the major common sequencing strategies and algo-rithms, their advantages and disadvantages, and the corresponding solutions. Finally, we envision the developing trends of high throughput sequencing technology, especially the third generation sequencing technology, and its ap-plication in transposon studies in the future, hopefully providing a comprehensive understanding and reference for related scientific researchers.