CAJ | 학술논문

目的：利用微生物转化方法构建制备20（S）-原人参三醇衍生物的新合成途径，并对转化条件进行优化，提高转化产物的产率。方法选取黑曲霉（Aspergillus niger AS 3.1858）为转化菌株，从接菌量、底物浓度、转化时间、加样时间、温度、转速等方面对转化条件进行优化。结果获得2个20（S）-原人参三醇衍生物，即24-亚甲基-20（S）-原人参三醇和23，24-烯-25-甲氧基-20（S）-原人参三醇；并获得了优化的转化工艺，即接种量为15%、底物浓度为0.25 mmol/L、加样时间为转种后36 h、培养温度为26℃、转化时间为5 d、摇床转速为150 r/min。结论该转化方法能简便地获得20（S）-原人参三醇衍生物，采用优化后的转化条件，24-亚甲基-20（S）-原人参三醇和23，24-烯-25-甲氧基-20（S）-原人参三醇的产率均明显增大。
목적：이용미생물전화방법구건제비20（S）-원인삼삼순연생물적신합성도경，병대전화조건진행우화，제고전화산물적산솔。방법선취흑곡매（Aspergillus niger AS 3.1858）위전화균주，종접균량、저물농도、전화시간、가양시간、온도、전속등방면대전화조건진행우화。결과획득2개20（S）-원인삼삼순연생물，즉24-아갑기-20（S）-원인삼삼순화23，24-희-25-갑양기-20（S）-원인삼삼순；병획득료우화적전화공예，즉접충량위15%、저물농도위0.25 mmol/L、가양시간위전충후36 h、배양온도위26℃、전화시간위5 d、요상전속위150 r/min。결론해전화방법능간편지획득20（S）-원인삼삼순연생물，채용우화후적전화조건，24-아갑기-20（S）-원인삼삼순화23，24-희-25-갑양기-20（S）-원인삼삼순적산솔균명현증대。
Objective To construct a new synthetic route to prepare 20 (S)-protopanaxatriol by using the microbial transformation method,to optimize the transformation condition for increasing the yield rate of the transformation products. Methods Aspergillus niger AS 3.1858 was selected as the transformation bacterial strain. The transformation conditions were op-timized in the aspects of inoculated bacterial quantity,substrate concentration,transformation time,sampling time,temperature and rotational speed. Results Two derivatives 24-methylene-20(S)-protopanaxatriol and 23,24-en-25-methoxyl-20(S)-pro-topanaxatriol were obtained;the optimized transformation technology was obtained ,the inoculation amount was15%,the substrate concentration was 0.25 mmol/L,the sample adding time was at 36 h after inoculation,the culture time was 26℃,the transforma-tion time was 5 d and the shaking speed was 15 r/min. Conclusion This microbial transformation method can simply obtain the derivatives of 20(S)-protopanaxatriol. The yield rate of 24-methylene-20(S)-protopanaxatriol and 23,24-en-25-methoxyl-20(S)-protopanaxatriol could be greatly increased by adopting the optimized condition.