CAJ | 학술논문

目的：探究眼镜蛇长链神经毒素（CBT）的镇痛作用及可能机制。方法采用在体细胞外记录方法记录慢性炎症痛模型大鼠脊髓背角神经元的痛诱发放电，分别对六组实验大鼠注射生理盐水（5μL）、CBT（20、40、80 ng/kg）、阿托品（2 mg/kg）、阿托品（2 mg/kg）+CBT（40 ng/kg），观察不同含量CBT对脊髓背角神经元痛诱发放电的影响，同时观察胆碱能受体拮抗剂阿托品能否翻转其镇痛效应。结果不同含量CBT均可以显著抑制慢性炎症痛大鼠脊髓背角神经元的痛诱发放电，各含量CBT组放电频率与生理盐水组比较，差异均有统计学意义（P＜0.05），且该效应可被阿托品所阻断，阿托品+CBT组在20、30、40、50、60 min 放电频率与CBT（40 ng/kg）组比较，差异均有统计学意义（P＜0.05）。结论CBT具有显著的镇痛效应，该效应有一定的剂量依赖性，胆碱能受体系统参与了其镇痛过程。
목적：탐구안경사장련신경독소（CBT）적진통작용급가능궤제。방법채용재체세포외기록방법기록만성염증통모형대서척수배각신경원적통유발방전，분별대륙조실험대서주사생리염수（5μL）、CBT（20、40、80 ng/kg）、아탁품（2 mg/kg）、아탁품（2 mg/kg）+CBT（40 ng/kg），관찰불동함량CBT대척수배각신경원통유발방전적영향，동시관찰담감능수체길항제아탁품능부번전기진통효응。결과불동함량CBT균가이현저억제만성염증통대서척수배각신경원적통유발방전，각함량CBT조방전빈솔여생리염수조비교，차이균유통계학의의（P＜0.05），차해효응가피아탁품소조단，아탁품+CBT조재20、30、40、50、60 min 방전빈솔여CBT（40 ng/kg）조비교，차이균유통계학의의（P＜0.05）。결론CBT구유현저적진통효응，해효응유일정적제량의뢰성，담감능수체계통삼여료기진통과정。
Objective To investigate the analgesic effect of cobratoxin (CBT) and its possible mechanisms. Methods The pain-evoked discharge from the spinal dorsal horn neurons in rats with chronic pain was recorded by the somatic extracellular record method. The 6 experimental rat groups were injected by the normal saline (5μL),CBT(20,40,80 ng/kg),atropine(2 mg/kg) and atropine(2 mg/kg)+CBT(40 ng/kg) respectively. The influence of different concentrations of CBT on the pain-evoked discharge from the spinal dorsal horn neurons was observed. At the same time ,whether cholinergic receptor antagonist atropine overturning its analgesic effect was observed too. Results The different concentrations of CBT could obviously inhibit the pain-evoked discharge from the spinal dorsal horn neuron in rats with chronic pain ,the discharge frequency had statistically significant difference between the different concentration and the normal saline group (P<0.05),moreover the effect could be blocked by atropine , the difference of the discharge frequency at 20,30,40,50,60 min had statistical difference between the atropine+CBT group and the CBT 40 ng/kg group(P<0.05). Conclusion CBT possesses a significant analgesic effect,this effect has certain dose dependence and the cholinergic receptor system participates in this analgesic process.