CAJ | 학술논문

硼酸受体识别体系近年来被广泛用于糖的分子识别研究,硼酸在一定酸度条件下,可特异性结合含顺式邻位多羟基的物质(如二醇、糖等)。本实验利用EDC/NHS化学交联,3-氨基苯硼酸( APBA)与聚丙烯酸( PAA)反应,制得苯硼酸接枝的聚合物PAA-PBA,进而将此聚合物进行层层组装,获得苯硼酸功能化薄膜。利用石英晶体微天平( QCM)和原子力显微镜( AFM)分别从宏观和微观的角度监测分析了此薄膜表面对糖类化合物的识别性质。结果表明此类薄膜对一定浓度(>50μg/mL)的糖类化合物均能表现出明显识别,其中,对果糖识别最为明显。此体系抗干扰性好,可重复使用,长期保存后仍能较好保持性能。微观表面形貌观察发现糖类化合物在薄膜表面识别均匀,识别后薄膜表面粗糙度发生明显变化。本研究成果可望用于开发高选择性、高灵敏度的糖识别体系。
붕산수체식별체계근년래피엄범용우당적분자식별연구,붕산재일정산도조건하,가특이성결합함순식린위다간기적물질(여이순、당등)。본실험이용EDC/NHS화학교련,3-안기분붕산( APBA)여취병희산( PAA)반응,제득분붕산접지적취합물PAA-PBA,진이장차취합물진행층층조장,획득분붕산공능화박막。이용석영정체미천평( QCM)화원자력현미경( AFM)분별종굉관화미관적각도감측분석료차박막표면대당류화합물적식별성질。결과표명차류박막대일정농도(>50μg/mL)적당류화합물균능표현출명현식별,기중,대과당식별최위명현。차체계항간우성호,가중복사용,장기보존후잉능교호보지성능。미관표면형모관찰발현당류화합물재박막표면식별균균,식별후박막표면조조도발생명현변화。본연구성과가망용우개발고선택성、고령민도적당식별체계。
Boronic acids and their derivatives have been widely used in carbohydrate-sensitive materials because they can selectively bind 1,2-and 1,3-diol compounds, including sugars, to form cyclic boronate esters. In this work, pheylboronic acid ( PBA) moieties were grafted onto the backbone of poly( acrylic acid) ( PAA) through the condensation reaction between aminopheyl-boronic acid and carboxylic acid group of PAA in the presence of EDC/NHS, designed as PAA-PBA. Then the resulting PAA-PBA were assembled with poly ( ethyleneimine) ( PEI) to form PAA-PBA multilayer films. The sensing performance of the PEI/PAA-PBA film to carbohydrate (> 50 μg/mL ) , including glucose, fructose, mannose and galactose, has been investigated by combination of the complementary techniques of quartz crystal microbalance ( QCM ) and atomic force microscopy ( AFM) . It was demonstrated that the multilayer showed higher sensitivity to fructose than glucose, mannose and galactose. The interferences of ascorbic acid, uric acid and dopamine to the recognition of glucose can be avoided and the multilayer sensor with excellent long term stability can be recycled by changing pH value of buffer solutions. This system may be potential in realization of high selectivity and high sensitivity sensing system for probing carbohydrate.