CAJ | 학술논문

以Al2 O3纳米通道膜为基体制备金纳米通道,以场发射扫描电镜、循环伏安、交流阻抗等方法对金纳米通道进行表征。采用EDC-NHS的交联反应,将壳聚糖自组装至金纳米通道孔壁上,形成表面具有手性位点选择性的功能化纳米通道膜,利用纳米通道优异的分离能力手性分离D-,L-组氨酸。考察了纳米通道孔径和溶液的pH值对分离效果的影响。采用银溶胶作为表面增强拉曼( SERS)测试的基底,增强对D-,L-组氨酸的SERS效应,提高检测该物质的选择性和灵敏度。分别在1000和1590 cm-1处测定L-组氨酸和D-组氨酸。在含200μL组氨酸、100μL银溶胶和100μL 80 mmol/L NaCl溶液(pH=7.59)中,D-组氨酸和L-组氨酸可得到较好分离,分离度达到4.91。
이Al2 O3납미통도막위기체제비금납미통도,이장발사소묘전경、순배복안、교류조항등방법대금납미통도진행표정。채용EDC-NHS적교련반응,장각취당자조장지금납미통도공벽상,형성표면구유수성위점선택성적공능화납미통도막,이용납미통도우이적분리능력수성분리D-,L-조안산。고찰료납미통도공경화용액적pH치대분리효과적영향。채용은용효작위표면증강랍만( SERS)측시적기저,증강대D-,L-조안산적SERS효응,제고검측해물질적선택성화령민도。분별재1000화1590 cm-1처측정L-조안산화D-조안산。재함200μL조안산、100μL은용효화100μL 80 mmol/L NaCl용액(pH=7.59)중,D-조안산화L-조안산가득도교호분리,분리도체도4.91。
Gold nanochannels were prepared using Al2 O3 nanotubules membrane as the carrier and modified with chitosan by a classical N-(3-dimethylaminopropyl)-N-ethyl carbodiimide ( EDC)/N-hydroxysuccinimide ( NHS ) coupling reaction. The nanochannels were characterized by field emission scanning electron microscopy ( FESEM) , cyclic voltammetry and AC impedance method. The Au nanochannels modified with chitosan showed a chiral environment and can be used to separate histidine enantiomer. The effects of pore size and solution pH on the separation efficiency of histidine were investigated. To increase the detection sensitivity of D-, L-histidine, Ag nanoparticles were used to enhance the surface enhanced Raman scattering ( SERS) activity. The results showed that the chitosan-modified gold nanochannels can be used to separate chiral histidine based on this unique selective nanochannel membrane. L-Histidine and D-histidine were respectively detected by SERS at wavelengths of 1000 and 1590 cm-1 . The results showed that L-histidine and D-histidine were separated well in the mixture containing 200 μL of histidine, 100 μL of colloidal Ag and 100 μL of 80 mmol/L NaCl ( pH=7 . 59 ) with a separation efficiency of 4 . 91 .