山东医药
山東醫藥
산동의약
Shandong Medical Journal
2015年
40期
11-13
,共3页
温扬敏%万端静%谢永华%罗彩林%邱丹缨
溫颺敏%萬耑靜%謝永華%囉綵林%邱丹纓
온양민%만단정%사영화%라채림%구단영
食管肿瘤%榄绿粗叶木%一氧化氮%一氧化氮合酶%环鸟甘酸
食管腫瘤%欖綠粗葉木%一氧化氮%一氧化氮閤酶%環鳥甘痠
식관종류%람록조협목%일양화담%일양화담합매%배조감산
esophageal neoplasms%lasianthus lancilimbus%nitric oxide%nitric oxide synthase%cyclic guanosine monophosphate
目的：探讨榄绿粗叶木茎甲醇提取物（以下简称MESLL）对人食管癌EC－9706细胞增殖的影响及其机制。方法体外培养EC－9706细胞。①观察MESLL对细胞增殖的影响：取对数生长期EC－9706细胞，随机分为MESLL组、环磷酰胺组（CTX）组和空白组。 MESLL组分别给予0．01、0．05、0．1、0．2、0．4 mg／mL MESLL干预，CTX组给予1 mg／mL CTX干预，空白组不干预。干预24、48、72 h，检测各组细胞增殖抑制率。②观察MESLL对细胞NO活性及NOS、cGMP含量的影响：取对数生长期细胞，随机分为实验组及对照组。实验组分别置入含0．01、0．05、0．1、0．2、0．4 mg／mL MESLL的培养液培养48 h，对照组常规培养48 h。检测两组细胞上清液NO活性及细胞NOS、cGMP含量。结果①MESLL对细胞增殖的影响：干预24 h，仅MESLL组0．2、0．4 mg／mL细胞增殖抑制率高于CTX组（P均＜0．05）；干预48、72 h，MESLL组细胞增殖抑制率随MESLL浓度升高而增加，MESLL组0．1、0．2、0．4 mg／mL细胞增殖抑制率均高于CTX组（P均＜0．05）。②MESLL对细胞NO活性及NOS、cGMP含量的影响：随MESLL浓度增加，EC－9706细胞NO活性及NOS、cGMP含量呈降低趋势（P＜0．05）。结论 MESLL能抑制食管癌EC－9706细胞增殖，其机制可能与抑制NO／NOS－cGMP通路有关。
目的：探討欖綠粗葉木莖甲醇提取物（以下簡稱MESLL）對人食管癌EC－9706細胞增殖的影響及其機製。方法體外培養EC－9706細胞。①觀察MESLL對細胞增殖的影響：取對數生長期EC－9706細胞，隨機分為MESLL組、環燐酰胺組（CTX）組和空白組。 MESLL組分彆給予0．01、0．05、0．1、0．2、0．4 mg／mL MESLL榦預，CTX組給予1 mg／mL CTX榦預，空白組不榦預。榦預24、48、72 h，檢測各組細胞增殖抑製率。②觀察MESLL對細胞NO活性及NOS、cGMP含量的影響：取對數生長期細胞，隨機分為實驗組及對照組。實驗組分彆置入含0．01、0．05、0．1、0．2、0．4 mg／mL MESLL的培養液培養48 h，對照組常規培養48 h。檢測兩組細胞上清液NO活性及細胞NOS、cGMP含量。結果①MESLL對細胞增殖的影響：榦預24 h，僅MESLL組0．2、0．4 mg／mL細胞增殖抑製率高于CTX組（P均＜0．05）；榦預48、72 h，MESLL組細胞增殖抑製率隨MESLL濃度升高而增加，MESLL組0．1、0．2、0．4 mg／mL細胞增殖抑製率均高于CTX組（P均＜0．05）。②MESLL對細胞NO活性及NOS、cGMP含量的影響：隨MESLL濃度增加，EC－9706細胞NO活性及NOS、cGMP含量呈降低趨勢（P＜0．05）。結論 MESLL能抑製食管癌EC－9706細胞增殖，其機製可能與抑製NO／NOS－cGMP通路有關。
목적：탐토람록조협목경갑순제취물（이하간칭MESLL）대인식관암EC－9706세포증식적영향급기궤제。방법체외배양EC－9706세포。①관찰MESLL대세포증식적영향：취대수생장기EC－9706세포，수궤분위MESLL조、배린선알조（CTX）조화공백조。 MESLL조분별급여0．01、0．05、0．1、0．2、0．4 mg／mL MESLL간예，CTX조급여1 mg／mL CTX간예，공백조불간예。간예24、48、72 h，검측각조세포증식억제솔。②관찰MESLL대세포NO활성급NOS、cGMP함량적영향：취대수생장기세포，수궤분위실험조급대조조。실험조분별치입함0．01、0．05、0．1、0．2、0．4 mg／mL MESLL적배양액배양48 h，대조조상규배양48 h。검측량조세포상청액NO활성급세포NOS、cGMP함량。결과①MESLL대세포증식적영향：간예24 h，부MESLL조0．2、0．4 mg／mL세포증식억제솔고우CTX조（P균＜0．05）；간예48、72 h，MESLL조세포증식억제솔수MESLL농도승고이증가，MESLL조0．1、0．2、0．4 mg／mL세포증식억제솔균고우CTX조（P균＜0．05）。②MESLL대세포NO활성급NOS、cGMP함량적영향：수MESLL농도증가，EC－9706세포NO활성급NOS、cGMP함량정강저추세（P＜0．05）。결론 MESLL능억제식관암EC－9706세포증식，기궤제가능여억제NO／NOS－cGMP통로유관。
Objective To investigate the inhibitory effect of methanol extract from lasianthus lancilimbus ( MESLL) on proliferation of human esophagus carcinoma cells EC-9706 and the mechanism.Methods EC-9706 cells were cultured in vitro.①Observing the effect of MESLL on cell proliferation:the cells in the logarithmic growth phase were divided into MESLL group, cyclophosphamide ( CTX) group and control group.MESLL group was respectively treated with 0.01, 0.05, 0.1, 0.2 and 0.4 mg/mL MESLL, the CTX group was intervened by 1 mg/mL CTX and the control group was not treated.The proliferation inhibiting rates were detected after 24, 48 and 72 h.②Observing the effect of MESLL on NO ac-tivity and the levels of NOS and cGMP:the cells in the logarithmic growth phase were divided into the experimental group and the control group.The experimental group was respectively treated with 0.01, 0.05, 0.1, 0.2 and 0.4 mg/mL MESLL for 48 h, and the control group was not treated.The NO activity and the levels of NOS and cGMP were measured. Results ①After 24 h, the proliferation inhibiting rates treated with 0.2 mg/mL and 0.4 mg/mL MESLL were higher than those of the CTX group (all P<0.05).After 48 and 72 h, tumor inhibiting rate of MESLL group was increased with the increased concentrations of MESLL.The tumor inhibiting rates treated with 0.1 mg/mL, 0.2 mg/mL and 0.4 mg/mL MESLL were higher than those of the CTX group (all P<0.05).②Effect of MESLL on NO activity and the levels of NOS and cGMP:the activity of NO, the levels of NOS and cGMP reduced with the increased concentrations of MESLL ( P<0.05).Conclusions MESLL could inhibit the proliferation of EC-9706 cells, and the mechanism may be related with the inhibition of NO/NOS-cGMP pathway.
