CAJ | 학술논문

目的：观察热休克蛋白90抑制剂17-AAG联合顺铂（ DDP）对人肝癌HepG2细胞增殖的影响，并探讨其机制。方法将对数生长期的HepG2细胞分为对照组和A、B、C组。对照组只加培养基。 A组加入0．625μmol／L的17-AAG，B组加入1．0μg／mL的DDP，C组加入0．625μmol／L的17-AAG和1．0μg／mL的DDP。培养24、48 h后用MTT法测算A、B、C组细胞增殖抑制率，用流式细胞术测算各组细胞凋亡率，用RT-PCR法检测各组Bax、Bcl-2 mRNA。结果 A、B、C组培养48 h时细胞增殖抑制率均高于培养24 h时，P均＜0．05；培养时间相同时C组细胞增殖抑制率高于A、B组，P均＜0．05。培养48 h后A、B、C组细胞凋亡率和Bax mRNA均高于对照组，Bcl-2 mRNA低于对照组，P均＜0．05；C组细胞凋亡率和Bax mRNA均高于A、B组，Bcl-2 mRNA低于A、B组，P均＜0．05。结论17-AAG对人肝癌HepG2细胞具有生长抑制作用，其与顺铂联合有协同作用。其机制可能与上调细胞Bax mRNA、下调Bcl-2 mRNA表达有关。
목적：관찰열휴극단백90억제제17-AAG연합순박（ DDP）대인간암HepG2세포증식적영향，병탐토기궤제。방법장대수생장기적HepG2세포분위대조조화A、B、C조。대조조지가배양기。 A조가입0．625μmol／L적17-AAG，B조가입1．0μg／mL적DDP，C조가입0．625μmol／L적17-AAG화1．0μg／mL적DDP。배양24、48 h후용MTT법측산A、B、C조세포증식억제솔，용류식세포술측산각조세포조망솔，용RT-PCR법검측각조Bax、Bcl-2 mRNA。결과 A、B、C조배양48 h시세포증식억제솔균고우배양24 h시，P균＜0．05；배양시간상동시C조세포증식억제솔고우A、B조，P균＜0．05。배양48 h후A、B、C조세포조망솔화Bax mRNA균고우대조조，Bcl-2 mRNA저우대조조，P균＜0．05；C조세포조망솔화Bax mRNA균고우A、B조，Bcl-2 mRNA저우A、B조，P균＜0．05。결론17-AAG대인간암HepG2세포구유생장억제작용，기여순박연합유협동작용。기궤제가능여상조세포Bax mRNA、하조Bcl-2 mRNA표체유관。
Objective To explore the effects of 17-AAG combined with cisplatin ( DDP) on the proliferation of human hepatocellular carcinoma HepG 2 cells and its mechanism .Methods HepG2 cells in the logarithmic phase were divided into the control group and groups A , B and C.The control group was only added with medium .Group A was added with 0.625 μmol/L 17-AAG, group B was added with 1.0 μg/mL DDP, and group C was added with 0.625 μg/mL 17-AAG and 1.0 μg/mL DDP.After being cultured for 24 h and 48 h, the cell proliferation inhibition rates of groups A , B and C were determined by MTT , the apoptosis rate was measured by flow cytometry , and the Bax and Bcl-2 mRNA in each group was measured by RT-PCR.Results The cell proliferation inhibition rates of groups A , B and C which were cultured for 48 h were all higher than those of being cultured for 24 h (all P<0.05), the cell proliferation inhibition rate of group C was higher than those of groups A and B at the same time of culture (all P<0.05).After being cultured for 48 h, the apopto-sis rate and Bax mRNA in the groups A , B and C were higher than that of the control group , while Bcl-2 mRNA was lower than that of the control group (all P<0.05); the apoptosis rate and Bax mRNA of group C were higher than those of groupsAandB,buttheBcl-2mRNAwaslowerthanthoseofgroupsAandB(allP<0.05).Conclusions 17-AAGin-hibits the growth of human hepatocellular carcinoma HepG 2 cells and has synergistic effect with cisplatin .The mechanism may be related to the up-regulation of Bax mRNA and down-regulation of Bcl-2 mRNA expression .