山东医药
山東醫藥
산동의약
Shandong Medical Journal
2015年
39期
16-18
,共3页
七氟醚%肝缺血再灌注%肺损伤%动物实验模型
七氟醚%肝缺血再灌註%肺損傷%動物實驗模型
칠불미%간결혈재관주%폐손상%동물실험모형
sevoflurane%liver ischemia reperfusion%lung injury%animal experiment model
目的:观察七氟醚预处理对肝缺血再灌注致肺损伤的干预作用,并探讨其机制。方法健康Wistar大鼠30只,随机分为假手术组、模型组、七氟醚组,每组10只。模型组与七氟醚组制作部分肝缺血再灌注模型,其中七氟醚造模前给予七氟醚吸入30 min;假手术组仅游离肝门,但不阻断血供。于再灌注60 min时处死大鼠,测血清ALT、AST、TNF-α;取部分右肺上叶组织用于测算湿重与干重比值(W/D),部分制作石蜡切片用于病理观察;取左肺组织,测定丙二醛( MDA)及MMP-9 mRNA。结果光镜下假手术组肺组织结构未见异常;七氟醚组肺组织损伤程度较模型组减轻。模型组、七氟醚组血清ALT、AST、TNF-α水平升高,与假手术组相比,P均<0.05;七氟醚组血清ALT、AST、TNF-α水平低于模型组(P均<0.05)。模型组、七氟醚组肺组织W/D、MDA含量及MMP-9 mRNA相对表达量均升高,与假手术组相比,P均<0.05;七氟醚组肺组织W/D、MDA含量及MMP-9 mRNA相对表达量低于模型组(P均<0.05)。结论七氟醚可减轻肝缺血再灌注导致的肺损伤,机制可能与降低TNF-α水平、抑制MMP-9 mRNA表达等有关。
目的:觀察七氟醚預處理對肝缺血再灌註緻肺損傷的榦預作用,併探討其機製。方法健康Wistar大鼠30隻,隨機分為假手術組、模型組、七氟醚組,每組10隻。模型組與七氟醚組製作部分肝缺血再灌註模型,其中七氟醚造模前給予七氟醚吸入30 min;假手術組僅遊離肝門,但不阻斷血供。于再灌註60 min時處死大鼠,測血清ALT、AST、TNF-α;取部分右肺上葉組織用于測算濕重與榦重比值(W/D),部分製作石蠟切片用于病理觀察;取左肺組織,測定丙二醛( MDA)及MMP-9 mRNA。結果光鏡下假手術組肺組織結構未見異常;七氟醚組肺組織損傷程度較模型組減輕。模型組、七氟醚組血清ALT、AST、TNF-α水平升高,與假手術組相比,P均<0.05;七氟醚組血清ALT、AST、TNF-α水平低于模型組(P均<0.05)。模型組、七氟醚組肺組織W/D、MDA含量及MMP-9 mRNA相對錶達量均升高,與假手術組相比,P均<0.05;七氟醚組肺組織W/D、MDA含量及MMP-9 mRNA相對錶達量低于模型組(P均<0.05)。結論七氟醚可減輕肝缺血再灌註導緻的肺損傷,機製可能與降低TNF-α水平、抑製MMP-9 mRNA錶達等有關。
목적:관찰칠불미예처리대간결혈재관주치폐손상적간예작용,병탐토기궤제。방법건강Wistar대서30지,수궤분위가수술조、모형조、칠불미조,매조10지。모형조여칠불미조제작부분간결혈재관주모형,기중칠불미조모전급여칠불미흡입30 min;가수술조부유리간문,단불조단혈공。우재관주60 min시처사대서,측혈청ALT、AST、TNF-α;취부분우폐상협조직용우측산습중여간중비치(W/D),부분제작석사절편용우병리관찰;취좌폐조직,측정병이철( MDA)급MMP-9 mRNA。결과광경하가수술조폐조직결구미견이상;칠불미조폐조직손상정도교모형조감경。모형조、칠불미조혈청ALT、AST、TNF-α수평승고,여가수술조상비,P균<0.05;칠불미조혈청ALT、AST、TNF-α수평저우모형조(P균<0.05)。모형조、칠불미조폐조직W/D、MDA함량급MMP-9 mRNA상대표체량균승고,여가수술조상비,P균<0.05;칠불미조폐조직W/D、MDA함량급MMP-9 mRNA상대표체량저우모형조(P균<0.05)。결론칠불미가감경간결혈재관주도치적폐손상,궤제가능여강저TNF-α수평、억제MMP-9 mRNA표체등유관。
Objective To observe the intervention of sevoflurane pretreatment on rat pulmonary injury caused by liver ischemia reperfusion and to explore its mechanism .Methods A total of 30 healthy Wistar rats were randomly divided into sham-operation group , the model group and the sevoflurane group , with 10 rats in each group .Models of liver ischemia reperfusion were established in the model group and sevoflurane group , and rats of the sevoflurane group were given inhala-tion of sevoflurane for 30 min before modeling; while in the sham-operation group , only porta hepatis was separated but blood supply was not blocked .Rats were scarified at 60 min after reperfusion , and the serum alanine aminotransferase ( ALT) , aspertate aminotransferase ( AST) and tumor necrosis factor-α( TNF-α) were detected; part of right upper lobe was used to measure the wet/dry weight ratio ( W/D ratio) and part was used to make paraffin section for pathological ob-servation;and left lung tissue was taken to determine malondialdehyde ( MDA) and MMP-9 mRNA.Results Lung tissue of the sham-operation group was normal in structure under microscopy , while damage degree of lung tissue in the sevoflu-rane group was reduced as compared with that of the model group .Serum ALT, AST and TNF-αof the model group and sevoflurane group were increased as compared with those of the shan-operation group (all P<0.05); while serum ALT, AST and TNF-αof the sevoflurane group was lower than that of the model group (all P<0.05).W/D ratio, MDA content and relative expression of MMP-9 mRNA in the lung tissues of the model group and sevoflurane group were increased as compared with those of the sham-operation group ( all P<0.05 ); W/D ratio, MDA content and relative expression of MMP-9 mRNA in the lung tissues of the sevoflurane group were lower than those of the model group (all P<0.05).Con-clusion Sevoflurane can alleviate the pulmonary injury caused by liver ischemia reperfusion , whose mechanism may be re-lated to the lower levels of TNF-αand inhibition of MMP-9 mRNA expression, etc.