现代医药卫生
現代醫藥衛生
현대의약위생
Journal of Modern Medicine & Health
2015年
21期
3235-3237
,共3页
番泻叶(中药)%胶囊%番泻甙/分析%色谱法,高压液相
番瀉葉(中藥)%膠囊%番瀉甙/分析%色譜法,高壓液相
번사협(중약)%효낭%번사대/분석%색보법,고압액상
Cassiae angustifolia(TCD)%Capsules%Sennoside/analysis%Chromatography,high pressure liquid
目的：建立番泻总苷胶囊中番泻苷A、B含量的测定方法。方法采用高效液相色谱（HPLC）法，以八烷基硅烷键合硅胶柱、乙腈-0.1%三氟乙酸水溶液为流动相梯度洗脱，检测波长340 nm。结果番泻苷A在0.2104～1.0520μg、番泻苷B在0.2250～1.1250μg呈良好的线性关系，平均回收率分别为99.51%（RSD=1.30%）和99.07%（RSD=0.69%）。结论该方法简单快捷、结果可靠，可作为番泻总苷胶囊质量控制的方法。
目的：建立番瀉總苷膠囊中番瀉苷A、B含量的測定方法。方法採用高效液相色譜（HPLC）法，以八烷基硅烷鍵閤硅膠柱、乙腈-0.1%三氟乙痠水溶液為流動相梯度洗脫，檢測波長340 nm。結果番瀉苷A在0.2104～1.0520μg、番瀉苷B在0.2250～1.1250μg呈良好的線性關繫，平均迴收率分彆為99.51%（RSD=1.30%）和99.07%（RSD=0.69%）。結論該方法簡單快捷、結果可靠，可作為番瀉總苷膠囊質量控製的方法。
목적：건립번사총감효낭중번사감A、B함량적측정방법。방법채용고효액상색보（HPLC）법，이팔완기규완건합규효주、을정-0.1%삼불을산수용액위류동상제도세탈，검측파장340 nm。결과번사감A재0.2104～1.0520μg、번사감B재0.2250～1.1250μg정량호적선성관계，평균회수솔분별위99.51%（RSD=1.30%）화99.07%（RSD=0.69%）。결론해방법간단쾌첩、결과가고，가작위번사총감효낭질량공제적방법。
Objective To establish a method for the determination of sennoside A and B contents in Senna Total Glyco-sides Capsule. Methods The high performance liquid chromatography(HPLC) method was adopted,the gradient elution was performed with octadecyl silane bonded silica gel column ,the mobile phase of acetonitrile-0.1%trifluoroacetic acid aqueous solu-tion and the detection wavelength of 340 nm. Results Sennoside A in the range of 0.210 4-1.052 0μg and sennoside B in the range of 0.225 0-1.125 0μg had good linear relationship. The average recovery rates were 99.51%(RSD=1.30%) for sennoside A and 99.07%(RSD=0.69%) for sennoside B. Conclusion The established method is simple,quick and reliable,which can be used as a quality control method for Senna Total Glycosides Capsules.
