山东医药
山東醫藥
산동의약
Shandong Medical Journal
2015年
43期
34-35,36
,共3页
刘丽平%水丽君%龚瑞龙%曹江霞%黄秋香%张元珍
劉麗平%水麗君%龔瑞龍%曹江霞%黃鞦香%張元珍
류려평%수려군%공서룡%조강하%황추향%장원진
有核红细胞%胎儿有核红细胞%妊娠%孕妇%妊娠结局
有覈紅細胞%胎兒有覈紅細胞%妊娠%孕婦%妊娠結跼
유핵홍세포%태인유핵홍세포%임신%잉부%임신결국
nucleated red blood cells%fetus nucleated red blood cells%pregnancy%pregnant women%pregnancy outcome
目的：观察孕妇外周血胎儿有核红细胞计数的变化,探讨其在妊娠不良结局预测中的应用价值。方法收集87例孕12~18周孕妇的外周血,密度梯度离心法分离单个核细胞,采用PE-GPA /FITC-CD71单克隆抗体双色标记胎儿有核红细胞后进行流式细胞分析,追踪妊娠结局并进行分析。结果妊娠结局正常者外周血中胎儿有核红细胞百分比为0.89%±0.81%,妊娠结局不良者为3.25%±2.98%,两者相比,P<0.05。以胎儿有核红细胞百分比>3.79%作为截断值,预测妊娠不良结局的敏感度为76.9%、特异度为64.3%。结论与妊娠结局正常的孕妇相比,妊娠结局不良孕妇孕12~18周时外周血胎儿有核红细胞增多；对孕12~18周孕妇外周血胎儿有核红细胞进行计数有助于不良妊娠结局的预测,其敏感度及特异度均较高。
目的：觀察孕婦外週血胎兒有覈紅細胞計數的變化,探討其在妊娠不良結跼預測中的應用價值。方法收集87例孕12~18週孕婦的外週血,密度梯度離心法分離單箇覈細胞,採用PE-GPA /FITC-CD71單剋隆抗體雙色標記胎兒有覈紅細胞後進行流式細胞分析,追蹤妊娠結跼併進行分析。結果妊娠結跼正常者外週血中胎兒有覈紅細胞百分比為0.89%±0.81%,妊娠結跼不良者為3.25%±2.98%,兩者相比,P<0.05。以胎兒有覈紅細胞百分比>3.79%作為截斷值,預測妊娠不良結跼的敏感度為76.9%、特異度為64.3%。結論與妊娠結跼正常的孕婦相比,妊娠結跼不良孕婦孕12~18週時外週血胎兒有覈紅細胞增多；對孕12~18週孕婦外週血胎兒有覈紅細胞進行計數有助于不良妊娠結跼的預測,其敏感度及特異度均較高。
목적：관찰잉부외주혈태인유핵홍세포계수적변화,탐토기재임신불량결국예측중적응용개치。방법수집87례잉12~18주잉부적외주혈,밀도제도리심법분리단개핵세포,채용PE-GPA /FITC-CD71단극륭항체쌍색표기태인유핵홍세포후진행류식세포분석,추종임신결국병진행분석。결과임신결국정상자외주혈중태인유핵홍세포백분비위0.89%±0.81%,임신결국불량자위3.25%±2.98%,량자상비,P<0.05。이태인유핵홍세포백분비>3.79%작위절단치,예측임신불량결국적민감도위76.9%、특이도위64.3%。결론여임신결국정상적잉부상비,임신결국불량잉부잉12~18주시외주혈태인유핵홍세포증다；대잉12~18주잉부외주혈태인유핵홍세포진행계수유조우불량임신결국적예측,기민감도급특이도균교고。
Objective To observe the fetus nucleated red blood cell ( NRBC) count changes in the peripheral blood of pregnant women and to investigate its application in prediction of adverse pregnancy outcomes. Methods Blood samples were obtained from 87 pregnant women with the gestational age from 12 to 18 weeks, peripheral blood mononuclear cells were isolated by density gradient centrifugation and then we analyzed the cells marked with PE-GPA/FITC-CD71 mono-clonal antibodies on the flow cytometer. Tracking the pregnancy outcomes, and their correlation between fetus nucleated red blood cell measurement and adverse pregnancy outcomes was analyzed. Results The percentage of fetus NRBC in women with normal pregnancy outcomes was 0. 89% ± 0. 81%, 3. 25% ± 2. 98% in women with adverse pregnancy outcomes, and there was a significant difference between these two groups(P<0. 05). We took fetus NRBC percentage >3. 79% as the cutoff value to predict adverse outcomes of pregnancy, the sensitivity was 76. 9%, and the specificity was 64. 3%. Conclu-sions Compared with women with normal pregnancy outcomes, women with adverse pregnancy outcomes have a higher a-mount of fetus NRBC in the peripheral blood during the gestational age of 12-18 weeks. Measurement of fetus NRBC in ma-ternal peripheral blood has a predictive role in adverse pregnancy outcomes, with a higher sensitivity and specificity.
