CAJ | 학술논문

目的：观察钙激活性中电导钾离子通道(IKCa1)被阻断后对宫颈癌HeLa细胞增殖及IKCa1膜电位的影响。方法分别用IKCa1阻断剂克霉唑和RNA干扰法阻断HeLa细胞的IKCa1后,用RT-PCR技术检测HeLa细胞的IKCal mRNA,MTT法检测细胞OD值,膜片钳技术检测细胞IKCa1电流。结果克霉唑阻断IKCa1后,HeLa细胞的IKCa1 mRNA表达降低、IKCa1电流减弱、细胞 OD值下降,与对照组相比,P均<0.05。 RNA干扰法阻断IKCa1后,Hela细胞的IKCa1 mRNA表达下降、IKCa1电流减弱、细胞OD值降低,与空白对照组和阴性转染组相比, P均<0.05。结论阻断IKCa1能有效抑制HeLa细胞增殖,下调细胞IKCa1 mRNA的表达,降低其IKCa1电流；IKCa1通过调控HeLa细胞膜电位而影响其信号传递,调控HeLa细胞的增殖。
목적：관찰개격활성중전도갑리자통도(IKCa1)피조단후대궁경암HeLa세포증식급IKCa1막전위적영향。방법분별용IKCa1조단제극매서화RNA간우법조단HeLa세포적IKCa1후,용RT-PCR기술검측HeLa세포적IKCal mRNA,MTT법검측세포OD치,막편겸기술검측세포IKCa1전류。결과극매서조단IKCa1후,HeLa세포적IKCa1 mRNA표체강저、IKCa1전류감약、세포 OD치하강,여대조조상비,P균<0.05。 RNA간우법조단IKCa1후,Hela세포적IKCa1 mRNA표체하강、IKCa1전류감약、세포OD치강저,여공백대조조화음성전염조상비, P균<0.05。결론조단IKCa1능유효억제HeLa세포증식,하조세포IKCa1 mRNA적표체,강저기IKCa1전류；IKCa1통과조공HeLa세포막전위이영향기신호전체,조공HeLa세포적증식。
Objective To investigate the effects of blocking intermediate-conductance-Ca2+-activated K+ channels ( IKCa1) on the cell proliferation and the membrane potential changes of IKCa1 in cervical cancer HeLa cells. Methods IKCal of HeLa cells was blocked with IKCa1 channel inhibitor ( clotrimazole, CLT) and RNA interference, respectively. RT-PCR was used to detect the expression of IKCal mRNA in HeLa cells. The OD value of cells was detected by MTT, and IKCal current was detected by the patch clamp technique in HeLa cells. Results When IKCal was blocked with CLT in HeLa cells, the expression of IKCal mRNA was decreased, IKCal current wakened and the OD value of cells decreased as compared with that of the control group (all P<0. 05). When IKCal was blocked by small interfering RNA technology in HeLa cells, the expression of IKCal mRNA reduced, IKCal current wakened, and the OD value of cells decreased as com-pared with that of the blank group and negative transfection group (all P<0. 05). Conclusions Blocking IKCa1 could in-hibit cell proliferation, down-regualte the expression of IKCa1 mRNA and cut down the IKCal current in HeLa cells. IKCa1 could regulate the cell proliferation of HeLa cells by directly regulating the membrane potential and thus affecting its signal transmission.