CAJ | 학술논문

目的：观察miR-17-5p在宫颈癌组织中的表达变化及其对宫颈癌细胞生长和增殖的影响。方法用实时荧光定量PCR法对20例宫颈癌患者的癌组织和癌旁组织中的miR-17-5p进行检测。将人宫颈癌细胞株HeLa分组,分别转染人工构建的miR-17-5p质粒、阴性对照质粒、miR-17-5p的反义寡核苷酸质粒及无关序列寡核苷酸质粒,采用MTT法和平板克隆形成实验检测细胞生长活性和克隆数目。结果宫颈癌组织、癌旁组织中miR-17-5p的相对表达量分别为0.315±0.185、1.143±1.373,两者相比,P<0.05。与转染阴性对照质粒的HeLa细胞相比,转染miR-17-5p质粒的HeLa细胞生长活性、克隆形成率明显降低(P均<0.05)；与转染无关序列寡核苷酸质粒的HeLa细胞相比,转染miR-17-5p的反义寡核苷酸质粒的HeLa细胞生长活性、克隆形成率明显升高(P均<0.05)。结论宫颈癌组织中miR-17-5p表达减少,其可抑制宫颈癌细胞的生长和增殖。
목적：관찰miR-17-5p재궁경암조직중적표체변화급기대궁경암세포생장화증식적영향。방법용실시형광정량PCR법대20례궁경암환자적암조직화암방조직중적miR-17-5p진행검측。장인궁경암세포주HeLa분조,분별전염인공구건적miR-17-5p질립、음성대조질립、miR-17-5p적반의과핵감산질립급무관서렬과핵감산질립,채용MTT법화평판극륭형성실험검측세포생장활성화극륭수목。결과궁경암조직、암방조직중miR-17-5p적상대표체량분별위0.315±0.185、1.143±1.373,량자상비,P<0.05。여전염음성대조질립적HeLa세포상비,전염miR-17-5p질립적HeLa세포생장활성、극륭형성솔명현강저(P균<0.05)；여전염무관서렬과핵감산질립적HeLa세포상비,전염miR-17-5p적반의과핵감산질립적HeLa세포생장활성、극륭형성솔명현승고(P균<0.05)。결론궁경암조직중miR-17-5p표체감소,기가억제궁경암세포적생장화증식。
Objective To observe the expression changes of miR-17-5p in the cervical cancer tissues and its effects on cervical cancer cell growth and proliferation. Methods The levels of miR-17-5p in cervical cancer tissues and adjacent tissues of 20 patients were detected by real-time quantitative PCR. We divided the human cervical cancer cell line HeLa in-to different groups, which were respectively transfected by artificial miR-17-5p plasmid, negative control plasmid, miR-17-5p antisense oligonucleotide (ASO) plasmid and irrelevant sequence oligonucleotide plasmid. MTT and colony experiment were used to detect the growth activity and clone number. Results The relative expression of miR-17-5p in the cervical cancer tissues and adjacent tissues were 0. 315 ± 0. 185 and 1. 143 ± 1. 373 (P<0. 05). The cell growth and cloning effi-ciency of HeLa cells transfected with pri-miR-17-5p were significantly decreased as compared with those of HeLa cells transfected by negative control plasmid (all P<0. 05);the cell growth and cloning efficiency of HeLa cells transfected with pri-miR-17-5p ASO were significantly increased as compared with those of HeLa cells transfected by irrelevant sequence oli-gonucleotide plasmid (all P<0. 05). Conclusion The expression of miR-17-5p was significantly down-regulated in the cervical cancer tissues, which inhibited the cell growth and proliferation of cervical cancer cells.