现代中西医结合杂志
現代中西醫結閤雜誌
현대중서의결합잡지
Modern Journal of Integrated Traditional Chinese and Western Medicine
2015年
35期
3899-3901
,共3页
D2-40%淋巴管密度%高迁移率族蛋白B1%宫颈癌
D2-40%淋巴管密度%高遷移率族蛋白B1%宮頸癌
D2-40%림파관밀도%고천이솔족단백B1%궁경암
D2-40%lymphatic vessel density%HMGB1%cervical cancer
目的：探讨宫颈癌组织中淋巴管密度（LVD）与高迁移率族蛋白B1（HMGB1）表达的关系。方法采用免疫组化法检测86例宫颈癌患者正常组织与癌组织中的D2－40（ LVD特异性标记物）表达情况，计算组织LVD；用Western blot 法检测正常组织和癌组织中HMGB1表达情况，并进行定量分析；用Pearson相关分析法分析LVD与HMGB1之间的相关性。结果宫颈癌患者癌组织中LVD及HMGB1表达水平均显著高于正常组织（ P均＜0.05），Ⅲ＋Ⅳ期患者癌组织中LVD及HMGB1表达水平均显著高于Ⅰ＋Ⅱ期患者（ P均＜0.05）；癌组织中LVD与HMGB1表达水呈显著正相关关系（r＝0．97，P＜0．05）。结论宫颈癌组织中LVD与HMGB1的表达呈显著正相关关系，与肿瘤的发展密切相关，二者可能通过相互作用，共同促进肿瘤细胞的侵袭、转移等活动。
目的：探討宮頸癌組織中淋巴管密度（LVD）與高遷移率族蛋白B1（HMGB1）錶達的關繫。方法採用免疫組化法檢測86例宮頸癌患者正常組織與癌組織中的D2－40（ LVD特異性標記物）錶達情況，計算組織LVD；用Western blot 法檢測正常組織和癌組織中HMGB1錶達情況，併進行定量分析；用Pearson相關分析法分析LVD與HMGB1之間的相關性。結果宮頸癌患者癌組織中LVD及HMGB1錶達水平均顯著高于正常組織（ P均＜0.05），Ⅲ＋Ⅳ期患者癌組織中LVD及HMGB1錶達水平均顯著高于Ⅰ＋Ⅱ期患者（ P均＜0.05）；癌組織中LVD與HMGB1錶達水呈顯著正相關關繫（r＝0．97，P＜0．05）。結論宮頸癌組織中LVD與HMGB1的錶達呈顯著正相關關繫，與腫瘤的髮展密切相關，二者可能通過相互作用，共同促進腫瘤細胞的侵襲、轉移等活動。
목적：탐토궁경암조직중림파관밀도（LVD）여고천이솔족단백B1（HMGB1）표체적관계。방법채용면역조화법검측86례궁경암환자정상조직여암조직중적D2－40（ LVD특이성표기물）표체정황，계산조직LVD；용Western blot 법검측정상조직화암조직중HMGB1표체정황，병진행정량분석；용Pearson상관분석법분석LVD여HMGB1지간적상관성。결과궁경암환자암조직중LVD급HMGB1표체수평균현저고우정상조직（ P균＜0.05），Ⅲ＋Ⅳ기환자암조직중LVD급HMGB1표체수평균현저고우Ⅰ＋Ⅱ기환자（ P균＜0.05）；암조직중LVD여HMGB1표체수정현저정상관관계（r＝0．97，P＜0．05）。결론궁경암조직중LVD여HMGB1적표체정현저정상관관계，여종류적발전밀절상관，이자가능통과상호작용，공동촉진종류세포적침습、전이등활동。
Objective It is to investigate the relationship between the expression of lymphatic vessel density ( lymphatic vessel density,LVD) and high mobility group protein B1 (High mobility group protein box -1,HMGB1) in cervical cancer. Method s The expression of D2-40 ( LVD specific markers ) in normal tissues and tumor tissues of patients with cervical canc-er were detected by immunohistochemical method , LVD expression of HMGB1 in normal tissues and tumor tissues was detected by Blot Western , quantitative analysis;Correlation analysis between HMGB 1 and LVD was analyzed by pearson correlation . Resu lts The levels of LVD and HMGB1 in tumor tissues of patients with cervical cancer were significantly higher than that in normal tissues(P all<0.05), at the same time, the levels of LVD and HMGB1 in tumor tissues of patients with Ⅲ and Ⅳstage was significantly higher than that in ones with stage Ⅰand Ⅱ( P all<0.05); The expression level of LVD in cervical cancer tissues was positively correlated with the relative expression level of HMGB 1 protein(r=0.97,P<0.05).Conclusion The correlation of expression of LVD and HMGB 1 was significantly positive in cervical cancer , and the expression levels was closely correlated with tumor stage , they can work together to promote the invasion and metastasis of tumor cells .
