CAJ | 학술논문

为了确定十倍体长穗偃麦草(Thinopyrum ponticum,Liu & Wang)和六倍体中间偃麦草(Th.intermedium,[Host]Barkworth & Dewey)的基因组组成,根据野生一粒小麦(Triticum boeoticum)着丝粒自主型反转录转座子(CRW)序列设计特异引物,以二倍体拟鹅观草(Pseudoroegneria spicata,(A) L(o)ve)基因组 DNA为模板进行PCR扩增,筛选到一条St基因组着丝粒区相对特异反转录转座子的部分序列pStC1,长度为1.755 kb(GenBank登录号:FJ952565),其中有800 bp与小麦着丝粒反转录转座子(CRW)的LTR区高度同源,另有小部分片段与其外壳蛋白编码基因(gag)部分同源,并且包含一段富含AGCAAC碱基的重复序列.以pStC1为探针,对十倍体长穗偃麦草的FISH检测结果显示其基因组组成为两个St组3个E组(St_1St_2E~eE~bE~x);pStC1与中间偃麦草杂交时,不仅St基因组上有强烈的荧光信号,而且E基因组一些染色体的近着丝粒区域也有杂交信号,说明偃麦草属异源多倍体物种在其形成及进化过程中St与E基因组之间在着丝粒及近着丝粒相关区域可能存在协同进化.
위료학정십배체장수언맥초(Thinopyrum ponticum,Liu & Wang)화륙배체중간언맥초(Th.intermedium,[Host]Barkworth & Dewey)적기인조조성,근거야생일립소맥(Triticum boeoticum)착사립자주형반전록전좌자(CRW)서렬설계특이인물,이이배체의아관초(Pseudoroegneria spicata,(A) L(o)ve)기인조 DNA위모판진행PCR확증,사선도일조St기인조착사립구상대특이반전록전좌자적부분서렬pStC1,장도위1.755 kb(GenBank등록호:FJ952565),기중유800 bp여소맥착사립반전록전좌자(CRW)적LTR구고도동원,령유소부분편단여기외각단백편마기인(gag)부분동원,병차포함일단부함AGCAAC감기적중복서렬.이pStC1위탐침,대십배체장수언맥초적FISH검측결과현시기기인조조성위량개St조3개E조(St_1St_2E~eE~bE~x);pStC1여중간언맥초잡교시,불부St기인조상유강렬적형광신호,이차E기인조일사염색체적근착사립구역야유잡교신호,설명언맥초속이원다배체물충재기형성급진화과정중St여E기인조지간재착사립급근착사립상관구역가능존재협동진화.
To verify the genome components of Thinopyrum ponticum Liu & Wang and Th. intermedium [Host] Barkworth & Dewey, six specific primer pairs were designed according to the sequence of an autonomous centromeric retrotransposon of wheat (CRW) from Triticum boeoticum. Several DNA fragments were amplified by PCR from the genomic DNA of the diploid species Pseudoroegneria spicata A Love. After sequence alignment, a 1.755 kb fragment was obtained and named as pStCl (St genome centromere associated sequence, GenBank accession No. FJ952565). This fragment contained an 800 bp fragment highly homologous to the LTR region of autonomous CRW, a short fragment partial homology to the gag region of CRW, and an AGCAAC-rich tandem repeat. Fluorescent in situ hybridization (FISH) using pStC1 as a probe was carried out on the chromosomes of Th. ponticum, Th. intermedium and Triticum aestivum cv. Chinese Spring. Th. ponticum was proved to have two St and three E genomes (St_1St_2E~eE~bE~x). In Th. intermedium, strong FISH signals were observed on St genome chromosomes, while faint signals were also found on some .E-genome chromosomes at their pericentromeric regions. These results indicated that during speciation of sub-genomes in the allopolyploids of Thinopyrum genus, concerted evolution might have occurred at centromeric and pericentromeric regions.