CAJ | 학술논문

病害问题是制约我国扇贝养殖业发展的关键,因此贝类的先天免疫也成为当前研究的热点.丝氨酸蛋白酶是先天免疫中至关重要的酶类,在许多通路中起信号放大作用.目前对栉孔扇贝丝氨酸蛋白酶的研究主要集中于基因序列分析和表达谱研究,基因定位方面的研究尚未开展.文章以包含一种栉孔扇贝丝氨酸蛋白酶基因的BAC克隆为探针,利用BAC-FISH技术将其定位到一对同源染色体的长臂上,为丝氨酸蛋白酶基因的后续研究提供了基础;同时,利用PCR产物直接测序法筛选了该基因内部的6个SNP标记,这些SNP标记可供遗传图谱定位使用,从而可以实现遗传图谱与染色体间的锚定与初步整合.
병해문제시제약아국선패양식업발전적관건,인차패류적선천면역야성위당전연구적열점.사안산단백매시선천면역중지관중요적매류,재허다통로중기신호방대작용.목전대즐공선패사안산단백매적연구주요집중우기인서렬분석화표체보연구,기인정위방면적연구상미개전.문장이포함일충즐공선패사안산단백매기인적BAC극륭위탐침,이용BAC-FISH기술장기정위도일대동원염색체적장비상,위사안산단백매기인적후속연구제공료기출;동시,이용PCR산물직접측서법사선료해기인내부적6개SNP표기,저사SNP표기가공유전도보정위사용,종이가이실현유전도보여염색체간적묘정여초보정합.
Disease control is a main objective in the culture of Farter's scallop(Chalmys farreri).Many investigations have focused on the innate immune system of this species.Serine proteases amplify signals in many immune-related pathways and thus play a crucial role in innate immune system.Recently,studies on serine protease in C.farreri were mainly on sequence analysis and expression profile,but no study on gene localization was reported.In this study,a BAC clone (CFB040H03)that contains a sefine protease gene of C farreri was located on the long arms of a pair of homologous chromosomes by BAC-FISH technique.Six SNPs were found inside the serine protease gene using direct sequencing of the PCR products.The chromosomal localization of serine protease gene would provide fundamental support for further research on this gene.The SNPs obtained can be mapped to the genetic linkage map and is helpful to the construction of the integration map of C.farreri.