CAJ | 학술논문

目的检测不同B淋巴瘤细胞株中syk的表达情况,为分析B细胞淋巴瘤发生、发展及预后提供一定的理论基础. 方法采用RT-PCR法半定量检测Raji、Ramos和Namalwa 3种淋巴瘤细胞中syk基因水平的表达;采用免疫细胞化学法和Western blot方法检测3种B淋巴瘤细胞中Syk蛋白的表达.结果 Raji和Namalwa细胞syk mRNA(514bp)表达阳性,而Ramos细胞syk mRNA(514bp)表达阴性;免疫细胞化学结果显示,Raji和Namalwa细胞胞浆呈阳性染色,Ramos细胞胞浆未着色; Western blot印迹分析结果显示,Raji和Namalwa细胞检测到特异性Syk蛋白的表达,而Ramos细胞未检测到Syk蛋白的表达. 结论 Raji、Namalwa细胞syk基因和蛋白水平均表达阳性, Ramos细胞syk基因和蛋白水平均表达阴性.
목적 검측불동B림파류세포주중syk적표체정황,위분석B세포림파류발생、발전급예후제공일정적이론기출. 방법 채용RT-PCR법반정량검측Raji、Ramos화Namalwa 3충림파류세포중syk기인수평적표체;채용면역세포화학법화Western blot방법검측3충B림파류세포중Syk단백적표체.결과 Raji화Namalwa세포syk mRNA(514bp)표체양성,이Ramos세포syk mRNA(514bp)표체음성;면역세포화학결과현시,Raji화Namalwa세포포장정양성염색,Ramos세포포장미착색; Western blot인적분석결과현시,Raji화Namalwa세포검측도특이성Syk단백적표체,이Ramos세포미검측도Syk단백적표체. 결론 Raji、Namalwa세포syk기인화단백수평균표체양성, Ramos세포syk기인화단백수평균표체음성.
Objective Detect the expression of syk in various B lymphoma cells to provide theoretical basis for analysis the development and prognosis of B cell lymphoma. Methods The expression of syk mRNA in Raji,Ramos and Namalwa was examined by semi-quantitative RT-PCR technique; Using immunocytochemestry staining and western-blot to detect the expression of syk protein in 3 B lymphoma cells. Results Raji and Namalwa cells express syk (514bp) mRNA, but Ramos cells lack expression of syk mRNA. Immunocytochemistry staining results: the staining of syk was positive in both Raji and Namalwa cells and the buffy stain can be seen in endochylema, but the endochylema of Ramos cells has no the buffy stain. Western blot assay results: the specific expression of syk protein can be detected in both Raji and Namalwa cells but can not be detected in Ramos cells. Conclusions Expression of syk mRNA and protein is positive in both Raji and Namalwa cells, while, which is negative in Ramos cells.