CAJ | 학술논문

为研究阿片黑皮质素前体(POMC)在动物采食和能量平衡调控中发挥重要作用,文章采用PCR-SSCP结合DNA测序方法,对秦川牛、南阳牛、郏县红牛、晋南牛、鲁西牛,安格斯牛和荷斯坦奶牛共计480头个体POMC基因的多态性进行研究,并分析了多态位点与南阳牛生长性状的相关性.结果表明,牛POMC基因3个位点中,在3'侧翼区P3位点新发现3个连锁存在的SNP(811845 C＞T、811821 T＞C和811797 A＞G,与NW_928357对照).POMC基因3'侧翼区多态位点与南阳牛6月龄体重和0～6月龄平均日增重显著相关,BB型个体显著大于AA型(P＜0.05).
위연구아편흑피질소전체(POMC)재동물채식화능량평형조공중발휘중요작용,문장채용PCR-SSCP결합DNA측서방법,대진천우、남양우、겹현홍우、진남우、로서우,안격사우화하사탄내우공계480두개체POMC기인적다태성진행연구,병분석료다태위점여남양우생장성상적상관성.결과표명,우POMC기인3개위점중,재3'측익구P3위점신발현3개련쇄존재적SNP(811845 C＞T、811821 T＞C화811797 A＞G,여NW_928357대조).POMC기인3'측익구다태위점여남양우6월령체중화0～6월령평균일증중현저상관,BB형개체현저대우AA형(P＜0.05).
Pro-opiomelanocortin(POMC)plays an important role on animal ingestive behavior and energy homeostasis.Genetic variation of POMC was detected by PCR-SSCP and DNA sequencing techniques in 480 individuals from seven cattle breeds(i.e.,Qinchuan,Nanyang,Jiaxian Red cattle,Jinnan,Luxi,Angus,and Chinese Holstein),and association analyses were carried out to evaluate the effects of genotypes of the candidate genes on growth traits in Nanyang cattle.Three SNPs(811845 C＞T,811821 T＞C and 811797 A＞G,ref.NW_928357)in linkage were detected in P3 locus,which were located at the 3'flanking region of bovine POMC gene.The body weight at 6-month-old and the average daily gain of 0～6-month-old were higher in Nanyang cattle with the genotype BB than with the genotype AA (P＜0.05).