CAJ | 학술논문

提出了同时测定人血浆中的西诺沙星和萘啶酸的高效液相色谱方法。采用反相C18柱、254 nm紫外检测，乙腈-甲醇-0.01 mol/L草酸(30∶5∶65，V/V；pH 4.55)体系，实现了二者的良好分离。分别考察了磷酸缓冲溶液和草酸缓冲溶液作为流动相弱溶剂，结果草酸缓冲溶液能有效改善峰形，降低检测限。在不同pH值(pH 2.80,4.08,4.55)进行了实验，pH 4.55获得最佳分离。最低检测量分别为0.14 ng(西诺沙星)和0.24 ng(萘啶酸)。比文献报道的同类药物最低检测量低10倍。
제출료동시측정인혈장중적서낙사성화내정산적고효액상색보방법。채용반상C18주、254 nm자외검측，을정-갑순-0.01 mol/L초산(30∶5∶65，V/V；pH 4.55)체계，실현료이자적량호분리。분별고찰료린산완충용액화초산완충용액작위류동상약용제，결과초산완충용액능유효개선봉형，강저검측한。재불동pH치(pH 2.80,4.08,4.55)진행료실험，pH 4.55획득최가분리。최저검측량분별위0.14 ng(서낙사성)화0.24 ng(내정산)。비문헌보도적동류약물최저검측량저10배。
A method for the determination of cinoxacin and nalidixic acid in human plasma by reverse-phase high performance liquid chromatography is introduced. The single separation system involves a RP-C18 column, a ultraviolet detector at 254 mn and CH3CN-CH3OH-0.01 mol/L HOOCOOH (30:5:65;pH 4.55). The influence of two kind of buffer (phosphate buffer and oxalate buffer) in mobile phase on the separation were studied. Oxalate buffer can improve tailing peak and decrease detection limits. Three pH values (pH 2.80,4.08,4.55) were studied, pH 4.55 can give best separation result. The detection limits of cinoxacin and nalidixic acid were 0.14 ng and 0.24 ng, respectively.